Comportement du génome du virus de la PPA dans l’isolat CAM/82 après son passage chez le porc et dans des cultures de sa moelle osseuse

N.F. Ekue; P.J. Wilkinson

doi:10.19182/remvt.9665

The effect of passage in pigs and pig bone marrow cultures on the genome of the Cameroon isolate (CAM/82) of ASF virus

Authors

N.F. Ekue, P.J. Wilkinson

DOI:

https://doi.org/10.19182/remvt.9665

Keywords

Swine, viruses, African swine fever, Bone marrow, genomes, Biological analysis, Cameroon

Abstract

A study was carried out to determine the effect of passage in pigs and pig bone marrow cultures on the genome of the Cameroon isolate (CAM/82) of ASF virus. Restriction enzyme analysis and cross hybridizations of the restriction enzyme fragments with plasmid DNA clones of ASF virus showed that no changes were detected in the genome of the CAM/82 isolate of ASFV when the virus was passed either in pigs or pig bone marrow cultures. The results presented in this study do suggest that the ASFV genome is quite stable during pig-to-pig transmission of the virus both in natural and in experimental conditions. These results also provide further epidemiological evidence for the persistence of ASF in the country by continuous circulation of viruses in pig populations.

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Affiliations

N.F. Ekue IRAD - CMR
P.J. Wilkinson
no@email.com
Institute for Animal Health - GBR

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Abstract
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Published

1999-03-01

How to Cite

Ekue, N., & Wilkinson, P. (1999). The effect of passage in pigs and pig bone marrow cultures on the genome of the Cameroon isolate (CAM/82) of ASF virus. Revue d’élevage Et De médecine vétérinaire Des Pays Tropicaux, 52(3-4), 209–213. https://doi.org/10.19182/remvt.9665