New tick and insect cell line resources for vector-borne disease research from the Tick Cell Biobank

Catherine  Hartley; Jing Jing Khoo; Alistair Darby; Benjamin L. Makepeace; Lesley Bell-Sakyi

doi:10.19182/remvt.37774

Autores/as

C. Hartley, J.J. Khoo, A. Darby, B.L. Makepeace, L. Bell-Sakyi

DOI:

https://doi.org/10.19182/remvt.37774

Palabras clave

Línea celular, garrapata, mosquito, culicoide, flebotomo, mosca tsé-tsé, chinche triatomino

Resumen

Contexto: Las líneas celulares de artrópodos tienen un papel importante en la investigación sobre el control de patógenos de transmisión vectorial con impacto veterinario, médico y agrícola. El Biobanco de Células de Garrapatas (TCB) de la Universidad de Liverpool es la única colección en el mundo de líneas celulares derivadas de garrapatas y de insectos vectores de patógenos virales, bacterianos, protozoarios y helmintos. Objetivo: El TCB de Liverpool y sus puestos avanzados en Malasia y Brasil se crearon para facilitar el acceso de los investigadores de todo el mundo a los recursos de líneas celulares de garrapatas y de insectos, así como para la formación en su mantenimiento, su aplicación y su desarrollo. Métodos: El TCB recibe y almacena líneas celulares de artrópodos y las distribuye a los científicos que las solicitan, también genera nuevas líneas celulares a partir de garrapatas y de insectos vectores. Para facilitar la adopción de líneas celulares, el TCB y sus puestos avanzados garantizan una formación en el cultivo de células de artrópodos y proporcionan consejos y apoyo permanente a los beneficiarios. Las líneas celulares se proporcionan en el marco de acuerdos de transferencia de material. Resultados: El TCB alberga actualmente más de 90 líneas celulares derivadas de garrapatas Ixodidae y Argasidae, de mosquitos, de mosquitas picadoras, de flebótomos, de moscas tsé-tsé, de triatominos y de abejas melíferas. Este artículo describe estos recursos nuevos y desarrollados/adquiridos recientemente, en especial las líneas celulares derivadas de las garrapatas Argas reflexus, Hyalomma lusitanicum, Hyalomma marginatum y Rhipicephalus bursa, así como insectos Anopheles stephensi, Apis mellifera, Culicoides sonorensis, Glossina morsitans, Phlebotomus argentipes y Triatoma infestans. Conclusiones: La mayoría de las especies de vectores representadas en la colección son de origen tropical o subtropical. Gracias a la distribución de estas líneas celulares existentes y nuevas, el TCB y sus puestos avanzados continuarán apoyando la investigación mundial sobre los vectores artrópodos y los agentes patógenos para el ganado y el hombre que transmiten.

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Afiliaciones

C. Hartley

Department of Infection Biology and Microbiomes, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, United Kingdom
J.J. Khoo

Department of Infection Biology and Microbiomes, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, United Kingdom
A. Darby Department of Infection Biology and Microbiomes, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, United Kingdom
B.L. Makepeace

Department of Infection Biology and Microbiomes, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, United Kingdom
L. Bell-Sakyi
L.Bell-Sakyi@liverpool.ac.uk

Department of Infection Biology and Microbiomes, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, United Kingdom

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