Studies on the origin of false positive reactions to the scro-diagnosis for contagious bovine pleuropneumonia
DOI:
https://doi.org/10.19182/remvt.8789Keywords
Cattle, Mycoplasma, Immunization, diagnosis, Immunological techniques, ELISAAbstract
Four groups of cattle were experimentally immunised by four myco plasma species of « mycoides-like » group, Mycoplasma (M) caprico lum, M. mycoides subsp. mycoides (LC), M. mycoides subsp. capri and M. species group 7 of LEACH (PGS0). They were then bled weekly during 2 months to establish antibodies kinetics against homologous and heterologous antigens. The standard method of complement fixation test (CFT) used in Europe and a new ELISA test for diagnosis of contagions bovine pleuropneumonia were performed in comparison with passive haemagglutination test (PHA) against antigens used for experimental immunisation. Cross reactions obtained are rather equal to the degree of similitude between these mycoplasma species. With CFT-cross reactions are transitory and occur only while homologous titers are very high, particularly with « PGS0 » and the two caprine mycoides strains. ELISA results using a threshold of positivity of optical density of 0.20, were similar to that obtained with CFT except ELISA specificity is not so different from CFT one. This experimental model could explain some natural situations.
Downloads
Downloads
-
Abstract195
-
PDF (Français)74
Received
Published
How to Cite
License
© F.Poumarat et al., hosted by CIRAD 1989
![Creative Commons License](http://i.creativecommons.org/l/by-nc-sa/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.