Characterization of virulence genes in Escherichia coli strains isolated from calves suffering from diarrhea in the Nikki commune of Benin

Authors

    K.C. Boko, K.P. Challaton, C.F.A. Salifou, N.O. Aguidissou, J.N. Duprez, D. Thiry, J.G. Mainil, S. Farougou

DOI:

https://doi.org/10.19182/remvt.37197

Keywords


Calves, digestive disorders, Enterobacteriaceae, pathogenicity, Benin

Abstract

Diarrhea is one of the main pathologies encountered on cattle farms in Benin. Calves, the first and most affected link in the chain. The aim of this study was to assess the presence of virulence genes in Escherichia coli strains likely to cause diarrhea in calves, and their resistance profiles to common antimicrobials. To this end, 106 calves were monitored for two months after their birth in the commune of Nikki. 33 fecal samples were directly collected from the rectums of 33 calves suffering from diarrhea and subjected to bacteriological analysis. All collected samples were positive for E. coli. Characterization of the isolated E. coli strains isolated for the presence of virulence genes and factors stx1, stx2, eae, sta, F41 and F5 revealed the presence of stx1 only, with a rate of 63.64%. E. coli strains carrying the stx1 gene showed resistance profiles to the most commonly used antibiotics in Beninese farms: tetracycline (70%), amoxicillin clavulanic acid (50%) and colistin (50%). The presence of the stx1 gene constitutes a source of contamination for animal products. Further studies are needed to perform serological typing and genome sequencing of E. coli strains. It would also be necessary to extend the sampling to other regions of Benin in order to better assess the status of farms with regard to E. coli strains isolated from calves and thus identify a possible zoonotic risk.

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Affiliations

  • K.C. Boko
    cyrilleboko@yahoo.fr
    Unité de recherche sur les maladies transmissibles, Laboratoire de recherches en biologie appliquée, Ecole polytechnique d’Abomey-Calavi, Université d’Abomey-Calavi, Cotonou, Bénin
  • K.P. Challaton
    Unité de recherche sur les maladies transmissibles, Laboratoire de recherches en biologie appliquée, Ecole polytechnique d’Abomey-Calavi, Université d’Abomey-Calavi, Cotonou, Bénin
  • C.F.A. Salifou Laboratoire de biotechnologie animale et de technologie des viandes, Département de production et santé animales, Ecole polytechnique d’Abomey-Calavi, Université d’Abomey-Calavi, Cotonou, Bénin
  • N.O. Aguidissou
    Unité de recherche sur les maladies transmissibles, Laboratoire de recherches en biologie appliquée, Ecole polytechnique d’Abomey-Calavi, Université d’Abomey-Calavi, Cotonou, Bénin
  • J.N. Duprez Service de bactériologie vétérinaire, Département des maladies infectieuses et parasitaires, Faculté de médecine vétérinaire, Université de Liège, Belgique
  • D. Thiry
    Service de bactériologie vétérinaire, Département des maladies infectieuses et parasitaires, Faculté de médecine vétérinaire, Université de Liège, Belgique
  • J.G. Mainil
    Service de bactériologie vétérinaire, Département des maladies infectieuses et parasitaires, Faculté de médecine vétérinaire, Université de Liège, Belgique
  • S. Farougou Unité de recherche sur les maladies transmissibles, Laboratoire de recherches en biologie appliquée, Ecole polytechnique d’Abomey-Calavi, Université d’Abomey-Calavi, Cotonou, Bénin

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Received

2023-06-27

Accepted

2024-03-28

Published

2024-06-05

How to Cite

Boko, K. C., Challaton, K. P., Salifou, C. F. A. ., Aguidissou, N. O., Duprez, J.-N., Thiry, D. ., Mainil, J. G. ., & Farougou, S. . (2024). Characterization of virulence genes in Escherichia coli strains isolated from calves suffering from diarrhea in the Nikki commune of Benin. Revue d’élevage Et De médecine vétérinaire Des Pays Tropicaux, 77, 1–6. https://doi.org/10.19182/remvt.37197

Issue

Vol. 77 (2024)

Section

Animal health and epidemiology

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© K.C.Boko et al., hosted by CIRAD 2024

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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