Kinetics of infestation by Haemonchus contortus and response of resistant (Martinik Black Belly) and susceptible (Lacaune) sheep breeds

Authors

    M. Benguesmia, M. Hamiroune, A. Chrétien, F. Prévot, C. Grisez, J.P. Bergeaud, C. Lacroux, C. Trumel, A. Geffre, K. Harhoura, M. Aissi, P. Jacquiet

DOI:

https://doi.org/10.19182/remvt.31874

Keywords


Lacaune, Martinik Black Belly sheep, Haemonchus contortus, resistance to anthelmintics, immune response, nematode infections, experimental infection

Abstract

The extension of resistance of gastrointestinal nematodes to anthelmintics, recently observed in Haemonchus contortus, represents a real problem and makes it urgent to find alternative solutions such as the selection of helminth-resistant animals. All cases of experimental infestations show significant individual
variability in egg excretion, allowing good discrimination between resistant and susceptible individuals, which enables selecting resistant individuals in the studied breeds. However, the lack of knowledge on immune response mechanisms in sheep worms is an obstacle to the development of this selection. It is known that the excretion level of parasite eggs in feces is a relevant indicator for assessing the resistance of an animal to worms. This criterion was examined in the present study carried out with two French sheep breeds, the Martinik Black Belly (resistant) and the Lacaune (sensitive). The sheep were infested orally with a single dose of 10,000 L3 larvae of H. contortus. The sheep response was monitored during antemortem and postmortem examinations. The results clearly showed that during a first H. contortus infestation there was a significant difference between the parasite populations (parasite intensity and egg production) and between certain pathophysiological parameters (blood and tissue eosinophilia) observed in the two sheep breeds.

Downloads

Download data is not yet available.

Affiliations

  • M. Benguesmia
    m.benguesmia@laposte.net
    1. Ecole nationale supérieure vétérinaire Rabie Bouchama, Alger, Algérie. 2. Ecole nationale vétérinaire de Toulouse, Toulouse, France.
  • M. Hamiroune Département des sciences agro-vétérinaires, Faculté des sciences de la nature et de la vie, Université Ziane Achour, Djelfa, Algérie.
  • A. Chrétien Ecole nationale vétérinaire de Toulouse, Toulouse, France.
  • F. Prévot Ecole nationale vétérinaire de Toulouse, Toulouse, France.
  • C. Grisez Ecole nationale vétérinaire de Toulouse, Toulouse, France.
  • J.P. Bergeaud Ecole nationale vétérinaire de Toulouse, Toulouse, France.
  • C. Lacroux Ecole nationale vétérinaire de Toulouse, Toulouse, France.
  • C. Trumel Ecole nationale vétérinaire de Toulouse, Toulouse, France.
  • A. Geffre Ecole nationale vétérinaire de Toulouse, Toulouse, France.
  • K. Harhoura Ecole nationale supérieure vétérinaire Rabie Bouchama, Alger, Algérie.
  • M. Aissi Ecole nationale supérieure vétérinaire Rabie Bouchama, Alger, Algérie.
  • P. Jacquiet Ecole nationale vétérinaire de Toulouse, Toulouse, France.

    References

    Aguerre S., Jacquiet P., Brodier H., Bournazel J.P., Grisez C., Prévot F., Michot L., et al., 2018. Resistance to gastrointestinal nematodes in dairy sheep: Genetic variability and relevance of artificial infection of nucleus rams to select for resistant ewes on farms. Vet. Parasitol., 256 (1): 16-23 DOI: https://doi.org/10.1016/j.vetpar.2018.04.004

    Amarante A.F.T., Bricarello P.A., Huntley J.F., Mazzolin L.P., Gomes J.C., 2005. Relationship of abomasal histology and parasite-specific immunoglobulin-A with the resistance to Haemonchus contortus infection in three breeds of sheep. Vet. Parasitol., 128 (1-2): 99-107 DOI: https://doi.org/10.1016/j.vetpar.2004.11.021

    Benguesmia M., 2010. Cinétiques des populations de parasites et des réponses de l’hôte au cours d’une primo-infestation par Haemonchus Contortus : comparaison des races Lacaune et Martinik Black Belly. Mém. Master 2 SAEPS, Université de Montpellier 2-Ecole nationale vétérinaire de Toulouse, France, 83 p.

    Bentounsi B., Zouiouech H., Benchikh-Elfegoun C., Kohil K., Cabaret J., 2003. Efficacité comparée des spécialités d’albendazole distribuées en Algérie. Rev. Méd. Vét., 154 (10) : 649-652

    Bishop S.C., Morris C.A., 2007. Genetics of disease resistance in sheep and goats. Small Rumin. Res., 70 (1): 48-59 DOI: https://doi.org/10.1016/j.smallrumres.2007.01.006

    Bordes L., Dumont N., Lespine A., Souil E., Sutra J.F., Prévot F., Grisez C., et al., 2020. First report of multiple resistance to eprinomectin and benzimidazole in Haemonchus contortus on a dairy goat farm in France. Parasitol. Int., 76: 102063 DOI: https://doi.org/10.1016/j.parint.2020.102063

    Boulkaboul A., 2008. Evaluation du parasitisme par les strongles digestifs et de l’efficacité du traitement anthelminthique chez les ovins dans la région de Tiaret. Thèse Doct., Université d’Oran Es-Senia, Algérie, 172 p.

    Bourdoiseau G.,1992. Résistance aux anthelminthiques. Point Vét., 147 : 13-20

    Dorny P., Vercruysse J., 1998. Evaluation of a micro method for the routine determination of serum pepsinogen in cattle. Res. Vet. Sci., 65 (3): 259-262 DOI: https://doi.org/10.1016/S0034-5288(98)90153-9

    Gonzalez J.F., Hernandez A., Molina J.M., Fernandez A., Raadsma H.W., Meeusen E.N.T., Piedrafita D., 2008. Comparative experimental Haemonchus contortus infection of two sheep breeds native to the Canary Islands. Vet. Parasitol., 153 (3-4): 374-378 DOI: https://doi.org/10.1016/j.vetpar.2008.02.019

    Gruner L., Aumont G., Getachew T., Brunel J.C., Pery C., Cognié Y., Guérin Y., 2003. Experimental infection of Black Belly and INRA 401 straight and crossbred sheep with trichostrongyle nematode parasites. Vet. Parasitol., 116 (3): 239-249 DOI: https://doi.org/10.1016/j.vetpar.2003.07.005

    Jacobsen E.A, Taranova A.G., Lee N.A., Lee J.J., 2007. Eosinophils: singularly destructive effector cells or purveyors of immunoregulation? J. Allergy Clin. Immunol., 119 (6): 1313-1320 DOI: https://doi.org/10.1016/j.jaci.2007.03.043

    Jacquiet P., Fidelle F., Grisez C., Prevot F., Lienard E., Bergeaud J.P., Sicard S., et al., 2011. Sélection sur phénotypes de la résistance aux strongles gastro-intestinaux en centre d’élevage de béliers. Renc. Rech. Rum., 18 : 343-346

    Kaplan R.M., Vidyashankar A.N., 2012. An inconvenient truth: global worming and anthelmintic resistance. Vet. Parasitol., 186 (1-2): 70-78 DOI: https://doi.org/10.1016/j.vetpar.2011.11.048

    Kotze A.C, Prichard R.K., 2016. Anthelmintic resistance in Haemonchus contortus. History, mechanisms and diagnosis. Adv. Parasitol., 93: 397-428 DOI: https://doi.org/10.1016/bs.apar.2016.02.012

    Lacroux C., 2006. Régulation des populations de nématodes gastro-intestinaux (Haemonchus contortus et Trichostrongylus colubriformis) dans deux races ovines, INRA 401 et Barbados Black Belly. Thèse Doct., Institut national polytechnique de Toulouse, France, 233 p.

    Lafri M., Ferrouk M., Harkat S., Routel A., Medkour M., Dasilva A., 2014 Caractérisation génétique des races ovines algériennes. In: Technology creation and transfer in small ruminants: roles of research, development services and farmer associations (Ed. Chentouf M., López-Francos A., Bengoumi M., Gabiña D.). CIHEAM, Options Méditerr. Sér. A., (108) : 293-298

    Mahieu M., Aumont G., Alexandre G., 1997. Elevage intensif des ovins tropicaux à la Martinique. Prod. Anim., 10 (1): 21-32 DOI: https://doi.org/10.20870/productions-animales.1997.10.1.3974

    Naves M., Alexandre G., Leimbacher F., Mandonnet N., Menendez-Buxadera A., 2001. Les ruminants domestiques de la Caraïbe : le point sur les ressources génétiques et leur exploitation. Prod. Anim., 14 (3) : 181-192 DOI: https://doi.org/10.20870/productions-animales.2001.14.3.3738

    Raynaud J.P., William G., Brunault G., 1970. Etude de l’efficacité d’une technique de coproscopie quantitative pour le diagnostic de routine et le contrôle des infestations parasitaires des bovins, équins et porcins. Ann. Parasitol. Hum. Comp., 45 (3) : 321-342 DOI: https://doi.org/10.1051/parasite/1970453321

    Rothenberg M.E., Hogan S.P., 2006. The eosinophil. Annu. Rev. Immunol., 24: 147-174 DOI: https://doi.org/10.1146/annurev.immunol.24.021605.090720

    Shakya K.P., Miller J.E., Horohov D.W., 2009. A TH2 type of immune response is associated with increased resistance to Haemonchus contortus in naturally infected Gulf Coast Native lambs. Vet. Parasitol., 163 (1-2): 57-66 DOI: https://doi.org/10.1016/j.vetpar.2009.03.052

    Stear M.J., Strain S., Bishop S.C., 1999. Mechanisms underlying resistance to nematode infection. Int. J. Parasitol., 29 (1): 51-56 DOI: https://doi.org/10.1016/S0020-7519(98)00179-9

    Terefe G., Lacroux C., Andreoletti O., Grisez C., Prévot F., Bergeaud J.P., Penicaud J., et al., 2007. Immune response to Haemonchus contortus infection in susceptible (INRA 401) and resistant (Barbados Black Belly) breeds of lambs. Parasite Immunol., 29 (8): 415-24 DOI: https://doi.org/10.1111/j.1365-3024.2007.00958.x

    Terefe G., Lacroux C., Prévot F., Grisez C., Bergeaud J.P., Bleuart C., Dorchies P., Foucras G., Jacquiet P., 2009. Eosinophils in Haemonchus contortus-infected resistant and susceptible breeds of sheep: Abomasal tissue recruitment and in vitro functional state. Vet. Parasitol., 165 (1-2): 161-164 DOI: https://doi.org/10.1016/j.vetpar.2009.06.041

©m.benguesmia

Downloads

Metrics
Views/Downloads
  • Abstract
    999
  • pdf (Français)
    412

Published

2020-06-29

How to Cite

Benguesmia, M., Hamiroune, M., Chrétien, A., Prévot, F., Grisez, C., Bergeaud, J.-P., Lacroux, C., Trumel, C., Geffre, A., Harhoura, K., Aissi, M., & Jacquiet, P. (2020). Kinetics of infestation by Haemonchus contortus and response of resistant (Martinik Black Belly) and susceptible (Lacaune) sheep breeds. Revue d’élevage Et De médecine vétérinaire Des Pays Tropicaux, 73(2), 123–131. https://doi.org/10.19182/remvt.31874

Issue

Vol. 73 No. 2 (2020)

Section

Animal health and epidemiology

Categories

License

© M.Benguesmia et al., hosted by CIRAD 2020

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Crossref
Scopus
Google Scholar
Europe PMC

Most read articles by the same author(s)