Identification of Cryptosporidium parvum IIa and IId zoonotic subtype families and Cryptosporidium bovis from calves in Algeria

Authors

    L. Sahraoui, M. Mammeri, M. Thomas, A. Chevillot, B. Polack, I. Vallée, J. Follet, H. Ain-Baaziz, K.T. Adjou

DOI:

https://doi.org/10.19182/remvt.37159

Keywords


Cattle, calves, Cryptosporidium, zoonoses, Algeria

Abstract

Cryptosporidiosis is a significant disease in calves caused by the parasitic protist Cryptosporidium. The infection results in severe symptoms such as diarrhea, dehydration, delayed growth, and weight loss, often leading to mortality and economic losses. This study aimed to detect Cryptosporidium spp. in fecal samples from calves in five Algerian provinces. A total of 65 fecal samples from calves were collected from 12 dairy cattle farms in the north-east of Algeria. The presence of the parasites was established by microscopic screening of the oocysts following an immunofluorescence assay (IFA). IFA-positive samples were analyzed by 18S rRNA PCR-RFLP (restriction fragment length polymorphism) to determine the species. Cryptosporidium parvum was subtyped by sequence analysis of the 60 kDa glycoprotein gene. Cryptosporidium oocysts were detected microscopically in 41/65 (63%) samples, of which 26/41 (63.4%) were positive by 18S rRNA PCR-RFLP. Two Cryptosporidium species were detected in 24 samples; C. parvum (20/24) and C. bovis (4/24). C. parvum isolates from IIa and IId zoonotic subtype families were detected: IIaA16G2R1 (9/24), IIdA16G1 (4/24), and IIaA15G2R1 (1/24). Thus, calves are reservoirs of zoonotic C. parvum subtypes and represent a public health concern.

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Affiliations

  • L. Sahraoui Anses, INRAE, Ecole Nationale Vétérinaire d’Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort, France. Laboratoire de production et santé animale, Ecole Nationale Supérieure Vétérinaire (ENSV) d'Alger, Issad-Abbes Oued-Smar, Algiers, Algeria
  • M. Mammeri
    Anses, INRAE, Ecole Nationale Vétérinaire d’Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort, France
  • M. Thomas
    Anses, INRAE, Ecole Nationale Vétérinaire d’Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort, France
  • A. Chevillot Anses, INRAE, Ecole Nationale Vétérinaire d’Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort, France
  • B. Polack
    Anses, INRAE, Ecole Nationale Vétérinaire d’Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort, France
  • I. Vallée
    Anses, INRAE, Ecole Nationale Vétérinaire d’Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort, France
  • J. Follet
    Université de Lille, CNRS, Centrale Lille, Junia, Université Polytechnique Hauts de France, UMR 8520 IEMN Institut d’Electronique de Microélectronique et de Nanotechnologie, Lille, France
  • H. Ain-Baaziz Laboratoire de production et santé animale, Ecole Nationale Supérieure Vétérinaire (ENSV) d'Alger, Issad-Abbes Oued-Smar, Algiers, Algeria
  • K.T. Adjou
    karim.adjou@vet-alfort.fr
    Anses, INRAE, Ecole Nationale Vétérinaire d’Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort, France

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© Karim Adjou, Vet Alfort

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Received

2023-05-15

Accepted

2023-08-28

Published

2023-11-15

How to Cite

Sahraoui, L., Mammeri, M., Thomas, M., Chevillot, A., Polack, B., Vallée, I., Follet, J., Ain-Baaziz, H., & Adjou, K. T. (2023). Identification of Cryptosporidium parvum IIa and IId zoonotic subtype families and Cryptosporidium bovis from calves in Algeria. Revue d’élevage Et De médecine vétérinaire Des Pays Tropicaux, 76, 1–6. https://doi.org/10.19182/remvt.37159

Issue

Vol. 76 (2023)

Section

Animal health and epidemiology

Categories

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© L.Sahraoui et al., hosted by CIRAD 2023

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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