New tick and insect cell line resources for vector-borne disease research from the Tick Cell Biobank

Catherine  Hartley; Jing Jing Khoo; Alistair Darby; Benjamin L. Makepeace; Lesley Bell-Sakyi

doi:10.19182/remvt.37774

Auteurs

C. Hartley, J.J. Khoo, A. Darby, B.L. Makepeace, L. Bell-Sakyi

DOI :

https://doi.org/10.19182/remvt.37774

Mots-clés

Lignée cellulaire, tique, moustique, moucheron, phlébotome, mouche tsétsé, punaise triatomine

Résumé

Contexte : Les lignées cellulaires d’arthropodes jouent un rôle important dans la recherche sur le contrôle des pathogènes à transmission vectorielle d’importance vétérinaire, médicale et agricole. La Tick Cell Biobank (TCB) de l’université de Liverpool est la seule collection au monde de lignées cellulaires dérivées de tiques et d’insectes vecteurs de pathogènes viraux, bactériens, protozoaires et helminthes. Objectif : Le TCB de Liverpool et ses avant-postes en Malaisie et au Brésil ont été créés pour faciliter l’accès des chercheurs du monde entier aux ressources en lignées cellulaires de tiques et d’insectes, ainsi que la formation à leur entretien, leur application et leur développement. Méthodes : Le TCB reçoit, stocke et distribue des lignées cellulaires d’arthropodes aux scientifiques qui en font la demande, et génère de nouvelles lignées cellulaires à partir de tiques et d’insectes vecteurs. Pour faciliter l’adoption des lignées cellulaires, le TCB et ses avant-postes assurent une formation à la culture des cellules d’arthropodes et fournissent des conseils et un soutien permanents aux bénéficiaires. Les lignées cellulaires sont fournies dans le cadre d’accords de transfert de matériel. Résultats : Le TCB abrite désormais plus de 90 lignées cellulaires dérivées de tiques ixodidae et argasidae, de moustiques, de moucherons piqueurs, de phlébotomes, de mouches tsé-tsé, de punaises triatomes et d’abeilles mellifères. Cet article décrit ces ressources nouvelles et récemment développées/acquises, notamment les lignées cellulaires dérivées des tiques Argas reflexus, Hyalomma lusitanicum, Hyalomma marginatum et Rhipicephalus bursa, et des insectes Anopheles stephensi, Apis mellifera, Culicoides sonorensis, Glossina morsitans, Phlebotomus argentipes et Triatoma infestans. Conclusions : La majorité des espèces de vecteurs représentées dans la collection sont d’origine tropicale ou subtropicale. Grâce à la distribution de ces lignées cellulaires existantes et nouvelles, le TCB et ses avant-postes continueront à soutenir la recherche mondiale sur les vecteurs arthropodes et les agents pathogènes pour le bétail et l’homme qu’ils transmettent.

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Affiliations

C. Hartley

Department of Infection Biology and Microbiomes, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, United Kingdom
J.J. Khoo

Department of Infection Biology and Microbiomes, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, United Kingdom
A. Darby Department of Infection Biology and Microbiomes, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, United Kingdom
B.L. Makepeace

Department of Infection Biology and Microbiomes, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, United Kingdom
L. Bell-Sakyi
L.Bell-Sakyi@liverpool.ac.uk

Department of Infection Biology and Microbiomes, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, United Kingdom

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